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raw western blot  (Protein Simple Inc)


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    Protein Simple Inc raw western blot
    Raw Western Blot, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/raw western blot/product/Protein Simple Inc
    Average 90 stars, based on 1 article reviews
    raw western blot - by Bioz Stars, 2026-03
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    Akk improves hepatic lipid metabolism via PI3K/Akt pathway in obese mice (A–C) Akk significantly affected mouse liver protein network interaction pathways, significantly regulated pathways and differential proteins by proteomic analysis. (D–J) <t>Western</t> <t>blot</t> in the PI3K/Akt pathway in mouse liver: p-PI3K/PI3K, p -AMPK/AMPK, p -Akt/Akt, PPARγ/GAPDH, and SREBP-1c/GAPDH. (K) mRNA levels of genes related to lipogenesis (ACC1, FASN, SCD1, and PPARγ), lipid oxidation (CPT1α), and lipid uptake (SREBP-1c) in mouse liver. <t>Data</t> are expressed as mean ± SD (n = 6–8), ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, compared with the Mod group, and p value was calculated using Dunnet’s post hoc test in one-way ANOVA.
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    Image Search Results


    Akk improves hepatic lipid metabolism via PI3K/Akt pathway in obese mice (A–C) Akk significantly affected mouse liver protein network interaction pathways, significantly regulated pathways and differential proteins by proteomic analysis. (D–J) Western blot in the PI3K/Akt pathway in mouse liver: p-PI3K/PI3K, p -AMPK/AMPK, p -Akt/Akt, PPARγ/GAPDH, and SREBP-1c/GAPDH. (K) mRNA levels of genes related to lipogenesis (ACC1, FASN, SCD1, and PPARγ), lipid oxidation (CPT1α), and lipid uptake (SREBP-1c) in mouse liver. Data are expressed as mean ± SD (n = 6–8), ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, compared with the Mod group, and p value was calculated using Dunnet’s post hoc test in one-way ANOVA.

    Journal: iScience

    Article Title: Akkermansia muciniphila ameliorates fatty liver through microbiota-derived α-ketoisovaleric acid metabolism and hepatic PI3K/Akt signaling

    doi: 10.1016/j.isci.2025.112458

    Figure Lengend Snippet: Akk improves hepatic lipid metabolism via PI3K/Akt pathway in obese mice (A–C) Akk significantly affected mouse liver protein network interaction pathways, significantly regulated pathways and differential proteins by proteomic analysis. (D–J) Western blot in the PI3K/Akt pathway in mouse liver: p-PI3K/PI3K, p -AMPK/AMPK, p -Akt/Akt, PPARγ/GAPDH, and SREBP-1c/GAPDH. (K) mRNA levels of genes related to lipogenesis (ACC1, FASN, SCD1, and PPARγ), lipid oxidation (CPT1α), and lipid uptake (SREBP-1c) in mouse liver. Data are expressed as mean ± SD (n = 6–8), ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, compared with the Mod group, and p value was calculated using Dunnet’s post hoc test in one-way ANOVA.

    Article Snippet: Raw data of Western Blot Data , This paper , Mendeley Data, https://doi.org/10.17632/3fzfd2npw8.1.

    Techniques: Western Blot

    α-ketoisovaleric acid is the key metabolite in improving hepatic lipid metabolism via PI3K/Akt pathway in obese mice (A) Flowchart of α-ketoisovaleric acid compensation experiment. All mice, except for the Con group, were fed an HFHCD from week 1 to week 8. α-ketoisovaleric acid group was orally administered α-ketoisovaleric acid every day, while the Con and Mod groups were given saline. (B) Representative photographs of mouse livers from each group. (C–H) Western blot in the PI3K/Akt pathway in mouse liver: p-PI3K/PI3K, p -AMPK/AMPK, p -Akt/Akt, PPARγ/GAPDH, and SREBP-1c/GAPDH. (I) mRNA levels of genes related to lipogenesis (ACC1, FASN, and PPARγ), lipid oxidation (CPT1α), and lipid uptake (SREBP-1c) in mouse liver. (J) Akk improves hepatic lipid metabolism via PI3K/Akt pathway through α-ketoisovaleric acid. Data are expressed as mean ± SD (n = 6–8), ∗ p < 0.05, ∗∗ p < 0.01, compared with the Mod group, and p value was calculated using Dunnet’s post hoc test in one-way ANOVA. Scale bars: 1 cm.

    Journal: iScience

    Article Title: Akkermansia muciniphila ameliorates fatty liver through microbiota-derived α-ketoisovaleric acid metabolism and hepatic PI3K/Akt signaling

    doi: 10.1016/j.isci.2025.112458

    Figure Lengend Snippet: α-ketoisovaleric acid is the key metabolite in improving hepatic lipid metabolism via PI3K/Akt pathway in obese mice (A) Flowchart of α-ketoisovaleric acid compensation experiment. All mice, except for the Con group, were fed an HFHCD from week 1 to week 8. α-ketoisovaleric acid group was orally administered α-ketoisovaleric acid every day, while the Con and Mod groups were given saline. (B) Representative photographs of mouse livers from each group. (C–H) Western blot in the PI3K/Akt pathway in mouse liver: p-PI3K/PI3K, p -AMPK/AMPK, p -Akt/Akt, PPARγ/GAPDH, and SREBP-1c/GAPDH. (I) mRNA levels of genes related to lipogenesis (ACC1, FASN, and PPARγ), lipid oxidation (CPT1α), and lipid uptake (SREBP-1c) in mouse liver. (J) Akk improves hepatic lipid metabolism via PI3K/Akt pathway through α-ketoisovaleric acid. Data are expressed as mean ± SD (n = 6–8), ∗ p < 0.05, ∗∗ p < 0.01, compared with the Mod group, and p value was calculated using Dunnet’s post hoc test in one-way ANOVA. Scale bars: 1 cm.

    Article Snippet: Raw data of Western Blot Data , This paper , Mendeley Data, https://doi.org/10.17632/3fzfd2npw8.1.

    Techniques: Saline, Western Blot